|dc.contributor.advisor||Desmulliez, Professor Marc||
|dc.contributor.author||Kavanagh, Deirdre M.||
|dc.description.abstract||Macro scale magnetic separation of pure biological particles from a complex biological
sample is a key technique performed in clinical and research settings. This thesis
focuses on the development of a microfluidic based magnetic separator for biological
applications. The work presented covers design, simulation, fabrication and testing of
the magnetic separator.
The magnetic separator design consists of a micron-sized channel fabricated in a
biocompatible polymer, containing one inlet and three outlets. Close to the channel wall
are soft permalloy elements. The external magnetic flux is provided by the permanent
magnets situated on either side of the channel. Theoretical aspects of the design are
discussed and special attention is paid to investigating the effects of the magnetic and
fluidic forces acting within the microdevice.
Fabrication of the magnetic separator was carried out in the Microsystems Engineering
Centre, Heriot Watt University and at Epigem Ltd., Redcar, U.K. The manufacturing
processes investigated include methods for rapid prototyping and UV-photolithography.
CO2 laser ablation and powder blasting of PMMA were investigated as rapid
prototyping techniques. Using UV-photolithography magnetic separators were realised
in PDMS and in SU-8. Soft permalloy elements were fabricated using UV-LIGA and
the correct permalloy ratio (Ni80Fe20) evaluated. Ultimately three magnetic separation
systems have been successfully fabricated based on the different fabrication approaches.
Magnetic separation on chip was successfully demonstrated for all three devices
fabricated. Flow cytometry a highly accurate method of particle counting and analysis
was used to verify the separation efficiency. Experimental testing results have shown
that magnetic and non-magnetic beads can be separated with high efficiency.||en_US
|dc.publisher||Eningeering and Physical Sciences||en_US
|dc.rights||All items in ROS are protected by the Creative Commons copyright license (http://creativecommons.org/licenses/by-nc-nd/2.5/scotland/), with some rights reserved.||
|dc.title||Microfluidic based magnetic separator for biological applications||en_US